Figure 7.

Relative mRNA quantification of reactive oxygen species- and vitamin D-associated genes. Effects of cisplatin or dacarbazine treatment on the mRNA levels of (A) SOD1, (B) SOD2, (C) CAT, (D) VDR, (E) PDIA3, (F) CYP2R1, (G) CYP3A4, (H) CYP27B1, (I) CYP24A1 and (J) CYP11A1 gene expression in human melanoma A375 cells pre-treated with 1,25(OH)₂D3. The cells were incubated with 100 nM 1,25(OH)₂D3 for 24 h, followed by exposure to 12 µM cisplatin or 6 µM dacarbazine for an additional 24 h. The mRNA levels were measured by reverse transcription-quantitative polymerase chain reactions. The data are presented as the mean ± standard deviation of 3 independent experiments carried out in duplicate. ^*P<0.05, ^**P<0.01 and ^***P<0.001, calculated using Student’s t-test vs. untreated control or between the two groups indicated by the bracket. 1α,25(OH)₂D3, 1α, 25-dihydroxyvitamin D3; SOD, superoxide dismutase; CAT, catalase; VDR, vitamin D receptor; PDIA3, protein disulfide-isomerase A3; CYP2R1, vitamin D 25-hydroxylase; CYP3A4, cytochrome P450 3A4; CYP27B1, 25-hydroxyvitamin D3 1-α-hydroxylase; CYP24A1, vitamin D 24-hydroxylase; CYP11A1, cholesterol side-chain cleavage enzyme.