Figure 6.

Effect of selected aspirin analogues on EGF (100 ng/ml) internalisation in the SW480 cell line at concentrations <0.5 mM. Confocal analysis of SW480 colorectal cancer cells incubated with Alexa Fluor 555-EGF and preincubated in the absence/presence of stated compound and vehicle control (0 mM) under HEPES-buffered conditions. Images were acquired at 405 nm for DAPI nucleic stain (blue) and 561 nm for Alexa Fluor-EGF (red). Representative images were captured at ×400 magnification (n=3). EGF, epidermal growth factor; F-DiA, fumaryldiaspirin; DIF, diflunisal.