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. 2019 Feb 1;54(4):1183–1194. doi: 10.3892/ijo.2019.4707

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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PLAC2 promotes proliferation and invasion of OSCC cells. (A) The transfection efficiency of knockdown or overexpression of PLAC2 was determined via reverse transcription-quantitative polymerase chain reaction. ^***P<0.001, compared with the control groups. (B) Cell viability was assessed with an MTT assay in OSCC cells. ^*P<0.05, compared with the control groups. (C) Ki-67 protein level was measured in OSCC cells with an immunofluorescence assay. (D and E) Cell migration and invasion abilities were assessed in OSCC cells using wound-healing and Matrigel assays, respectively. ^*P<0.05, ^***P<0.001, compared with the control groups. PLAC2, placenta-specific protein 2; OSCC, oral squamous cell carcinoma; si, small interfering; NC, negative control; p-NC, negative control overexpressed plasmid; p-PLAC2, PLAC2 overexpressed plasmid.