Table 2. The theoretical plate number, resolution, repeatability, tailing factor results.
Prednisolone | Megestrol acetate | Dexamethasone | Cortisone acetate | Hydroxyprogesterone | Diethylstillbestrol | |
Theoretical plate number | 6438.8 | 8654.2 | 6617.8 | 6821.8 | 6632.1 | 5828.7 |
Average peak areas±SD | 54729.8±0.769 | 346369.4±0.917 | 28830.6±1.398 | 33252.2±1.599 | 44405.2±1.460 | 1571.0±1.198 |
Resolution | 1.83 | 1.41 | 1.93 | 1.10 | 1.80 | ND |
The tail factor | 1.05 | 1.1 | 0.98 | 1.1 | 1.08 | 0.90 |
Continued: | ||||||
Nandrolone | Norethisterone | Progesterone | Testosterone propionate | Cortisone | Cortisol | |
Theoretical plate number | 6221.5 | 6482.6 | 8321.4 | 6328.1 | 6832.6 | 6651.2 |
Average peak areas±SD | 229107.2±0.173 | 43167.8±1.549 | 93432.4±1.549 | 122335.8±1.772 | 37132.0±1.984 | 87319.6±1.830 |
Resolution | 1.36 | ND | 1.18 | 1.60 | 1.75 | 1.34 |
The tail factor | 1.1 | 1.1 | 1.05 | 1.05 | 0.99 | 1.01 |
Continued: | ||||||
Aldosterone | Androsterone | Testosterone | Estradiol | Estriol | ||
Theoretical plate number | 6745.9 | 6432.8 | 7865.9 | 7634.8 | 6242.4 | |
Average peak areas±SD | 5247.8±0.426 | 11900.8±0.781 | 388847.4±0.644 | 1486.2±1.228 | 1910.2±0.678 | |
Resolution | 1.94 | ND | 1.60 | 1.56 | 1.13 | |
The tail factor | 0.90 | 0.92 | 1.05 | 0.92 | 1 |