Extended Data Figure 1. Nearly full-length antibody gene amplification from biological and technical replicate samples.

a) Schematic of biological and technical replicate samples. Biological replicates (columns) are derived from distinct cell aliquots, so identical clonotypes or sequences found in multiple biological replicates must arise from different cells. Technical replicates (rows) were amplified using discrete RNA aliquots from a single cell aliquot. b) Strategy for nearly full-length antibody heavy chains. Black arrows indicate primers. Primers in the cDNA synthesis step anneal to the heavy chain constant region (CH) and add the first unique molecular identifier (UMI) and the Illumina read 1 primer annealing site. Primers in the 2nd strand synthesis step anneal to the framework 1 (FR1) region of the variable gene and add a second UMI and the Illumina read 2 primer annealing site.