Skip to main content
. Author manuscript; available in PMC: 2019 Jul 21.
Published in final edited form as: Nature. 2019 Jan 21;566(7744):393–397. doi: 10.1038/s41586-019-0879-y

Extended Data Table 2. Primers used for antibody gene amplification.

Regions in parenthesis indicate “offset” positions. Random offset nucleotides are added in multiples of 2. Xs indicate the position of Illumina TruSeq indexes.

Name Sequence Step
lgM-RT ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNN(NNNNNN)GGTTGGGGCGGATGCACTCC RT
lgG-RT ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNN(NNNNNN)SGATGGGCCCTTGGTGGARGC RT
VH1-2SS AGACGTGTGCTCTTCCGATCT(NNNNNN)GGCCTCAGTGAAGGTCTCCTGCAAG 2nd strand synthesis
VH2-2SS AGACGTGTGCTCTTCCGATCT(NNNNNN)GTCTGGTCCTACGCTGGTGAACCC 2nd strand synthesis
VH3-2SS AGACGTGTGCTCTTCCGATCT(NNNNNN)CTGGGGGGTCCCTGAGACTCTCCTG 2nd strand synthesis
VH4-2SS AGACGTGTGCTCTTCCGATCT(NNNNNN)CTTCGGAGACCCTGTCCCTCACCTG 2nd strand synthesis
VH5-2SS AGACGTGTGCTCTTCCGATCT(NNNNNN)CGGGGAGTCTCTGAAGATCTCCTGT 2nd strand synthesis
VH6-2SS AGACGTGTGCTCTTCCGATCT(NNNNNN)TCGCAGACCCTCTCACTCACCTGTG 2nd strand synthesis
R1-fwd GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC PCR1
R1-rev ACACTCTTTCCCTACACGACG PCR1
R2-fwd CAAGCAGAAGACGGCATACGAGAGATCGGTCTCGGCATTCCTGCTGAAGATXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC PCR2
R2-rev AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG PCR2