Figure 2. Western blot validation of selected proteins in both the DKs8 and HT1080 EVs.

A. Western blot analysis of DKs8 and HT1080 EVs validating selected proteins (EphA2, EphB1, EphB4, Integrin α3, Integrin β8, Thrombospondin-1, ARRDC1, Claudin 3, hnRNP A1) identified in the iTRAQ study. B. Western blot analysis of DKs8 EVs validating hnRNP H3 identified in the iTRAQ study. C. Quantitation of Western blots from ≥3 independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 comparing the band intensities of the DKs8 or HT1080 LEVs and SEVs.