A. Western blot analysis of DKs8 shScramb. and shARRDC1-KD TCLs for ARRDC1 and Beta actin. B. Representative nanoparticle tracking traces of SEVs from DKs8 shScramb. and shARRDC1-KD cells. C. Quantitation of SEVs numbers from DKs8 shScramb. and shARRDC1–2 and −3 cells determined in nanoparticle tracking analysis (n=4). D. Western blot analysis of DKs8 shScramb. and shARRDC1-KD SEVs assessing the levels of EphA2, EphB1, EphB4, Integrin α3, Integrin β8, Thrombospondin-1, ARRDC1, Claudin 3 and Hsp70. E. Quantitation of Western blots from ≥3 independent experiments. * p < 0.05; *** p < 0.001 comparing the band intensities of DKs8 shScramb., shARRDC1-KD SEVs.