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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Pancreas. 2019 Mar;48(3):329–334. doi: 10.1097/MPA.0000000000001249

FIGURE 2.

FIGURE 2.

Cancer-associated fibroblasts induce EMT and facilitate PDAC migration. A, In vitro migration of Capan-1 cells following culture with CAF-conditioned or control media for 7 days. Data represent the mean and SD of 4 experiments. *P < 0.05, **P < 0.001, ***P < 0.0001. B, E-cadherin staining (red) of Capan-1 cells (GFP) following co-culture with CAFs for 7 days. Arrows indicate E-cadherin negative Capan-1 cells. C, The frequency of E-cadherin negative Capan-1 cells following culture with or without CAFs detected by flow cytometry. D, Relative mRNA expression of EMT associated genes in sorted Capan-1 cells following co-culture with CAFs. Data represent the mean and SD of 3 experiments.