Skip to main content
. Author manuscript; available in PMC: 2020 Mar 11.
Published in final edited form as: ACS Comb Sci. 2019 Jan 24;21(3):207–222. doi: 10.1021/acscombsci.8b00156

Figure 1. Ligand discovery methods.

Figure 1.

An affibody library and a fibronectin domain library were sorted for ligands that bound CD276 or Thy1 specifically. Libraries were sorted by five different schemes: 1) magnetic bead selection with recombinant extracellular domains followed by FACS with recombinant extracellular domains, 2) magnetic bead selection followed by FACS with detergent solubilized cell lysate, 3) magnetic bead selection followed by cell panning selection, 4) cell panning selection with magnetic bead depletion, and 5) cell panning selection.