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. Author manuscript; available in PMC: 2020 Mar 11.
Published in final edited form as: ACS Comb Sci. 2019 Jan 24;21(3):207–222. doi: 10.1021/acscombsci.8b00156

Figure 2. Enriched ligands evaluated for soluble extracellular domain and detergent-solubilized cell lysate binding.

Figure 2.

Yeast-displayed ligand populations were enriched for binders against CD276 and Thy1 using soluble extracellular domains immobilized on magnetic beads followed by FACS with either soluble extracellular domains or detergent-solubilized cell lysates. Binding specificity for each enriched population was assessed by comparison to negative controls. For FACS with soluble extracellular domains, yeast were labeled with 100 nM soluble CD276 or Thy1-Fc extracellular domains (blue) or irrelevant negative control proteins (300 nM streptavidin for CD276 or 100 nM human IgG for Thy1-Fc; orange) (A-D). For selection of ligands with detergent-solubilized cell lysates against CD276, yeast were labeled with MS1-CD276 lysate (blue) or MS1-Thy1 lysate (orange). For selection of ligands with detergent-solubilized cell lysates against Thy1, yeast were labeled with MS1-Thy1 lysate (blue) or MS1-CD276 lysate (orange) (E-H).