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. Author manuscript; available in PMC: 2020 Mar 11.
Published in final edited form as: ACS Comb Sci. 2019 Jan 24;21(3):207–222. doi: 10.1021/acscombsci.8b00156

Figure 7. Depletion of non-specific binders with mammalian cell pre-blocking.

Figure 7.

Mixtures of high-yield (A) or low-yield (B) CD276-specific, non-specific, and non-binding yeast were incubated with either CD276-negative disadhered MS1-Thy1 cells (mammalian cell pre-block) or buffer only (no depletion) followed by incubation with MS1-CD276 cell monolayers. Enrichment ratios for CD276-specific (black), non-specific (gray), or non-binding (white) clones are shown as the mean ± standard deviation of 6–12 trials. (C). Specific (black) or non-target-specific (grey) clones were subjected to selection with or without mammalian cell pre-blocking for a total of 13 clone. Each point represents the yield of a single well of selection using pre-blocking normalized by a corresponding well selected without pre-blocking. Each clone was panned either in duplicate or triplicate.