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. 2019 Feb 21;12(3):441–450. doi: 10.1016/j.stemcr.2019.01.018

Figure 2.

Figure 2

All GPC Populations Show Similar Glial Fate In Vivo while Improved Mice Show Increased Astrocytic Differentiation

(A) After transplantations, all GPCs integrated into various brain areas (left) and were capable of differentiating into astrocytes (middle) and oligodendrocytes (right).

(B) Analysis of cell fate showed that cells from all GPC populations differentiated into GFAP+ astrocytes and OLIG2+ oligodendrocytes.

(C) The number of donor cells was similar in 2-, 5-, and 9-month-old mice. The survival of PDGFαR+ cells after transplantation was significantly increased compared with A2B5+ and GLAST+ cells.

(D) Improved mice had a significantly higher percentage of injected cells (GFP+) that were GFAP+ compared with unimproved mice.

(E) Improved mice showed a higher average number of donor cells present in the corpus callosum and cerebellum, albeit not significantly.

In (B) and (C), A2B5+ n = 13 mice; GLAST+ n = 10 mice; PDGFαR+ n = 17 mice. In (D) and (E), improved n = 6 mice; unimproved n = 18 mice. (B) to (E) show mean ± SEM; ∗p < 0.05, ∗∗p < 0.01. Scale bars, 20 μm. See also Figures S1B and S1C.