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. 2019 Feb 21;12(3):597–610. doi: 10.1016/j.stemcr.2019.01.017

Figure 6.

Figure 6

Hyperglycemic iPSC-ECs Displayed Increased Susceptibility against Simulated Ischemia Reperfusion Injury that Can Be Prevented by Calpain Inhibition

(A) Post-reoxygenation cell viability of normoglycemia (NG) or hyperglycemia (HG) iPSC-ECs, with or without MDL-28170, following exposure to sIRI was determined by propidium iodide (PI) staining, as visualized by fluorescence microscopy. Data are represented as means ± SEM of four independent experiments, ∗p < 0.05 versus NG cells, #p < 0.05 versus HG cells. Scale bars, 100 μm.

(B) ATP measurement in NG- or HG-treated iPSC-ECs following post-reoxygenation, with or without MDL-28170. Data are represented as means ± SEM of four independent experiments, ∗p < 0.05 versus NG cells, #p < 0.05 versus HG cells.

(C) Measurement of ROS in NG- or HG-treated iPSC-ECs following sIRI, with and without MDL-28170. Data are represented as means ± SEM of four independent experiments, ∗p < 0.05 versus NG cells, #p < 0.05 versus HG cells.

(D) Measurement of caspase-3/7 activity in NG- or HG-treated iPSC-ECs following sIRI, with and without MDL-28170. Data are represented as means ± SEM of four independent experiments.