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. 2019 Feb 21;12(3):545–556. doi: 10.1016/j.stemcr.2019.01.019

Figure 3.

Figure 3

Effects of Kdm4a mRNA Injection (K) and/or Melatonin Treatment (M, 10 μM) on Apoptosis and ROS Levels in SCNT Embryos Using Cryopreserved Oocyte Cytoplasm

(A) Immunostaining of cloned blastocysts in the SCNT-CROC group after various treatments. The nuclei (blue) are stained with DAPI, and the TUNEL-positive apoptotic nuclei (green) are indicated by arrows. OCT4 staining (red) is indicated ICM. Scale bar, 100 μm.

(B) TUNEL-positive cells indicate each embryo with apoptotic cells. ∗∗p < 0.05, significantly different from SCNT-CROC group; ∗p < 0.05, significantly different from SCNT-CROC + K group (n = 4).

(C) ROS staining (green) of cloned morula in the SCNT-CROC group after various treatments. Scale bar, 100 μm.

(D) Quantification of ROS fluorescence intensity of cloned morula in the SCNT-CROC group after various treatments. The experiments were performed four times. In each replicate, n = 6–9 per group. The total number of embryos was 26 and 31 in the SCNT-CROC + K and SCNT-CROC + M + K groups, respectively. Asterisk indicates significantly different value (p < 0.05).