Table 1.
Effect of Kdm4a mRNA Injection on the Developmental Potential of Cloned Embryos from Cryopreserved Mouse Oocytes
| Group | mRNA (μg/μL) | No. of NT Oocytes | No. of 2-Cell Embryos (%)a | No. of 4-Cell Embryos (%)b | No. of 2-Cell Block Embryos (% ± SEM)b | No. of Blastocysts (% ± SEM)b |
|---|---|---|---|---|---|---|
| SCNT-FOC | – | 112 | 105 (94) | 70 (67) | 35 (33 ± 2.9)a | 27 (26 ± 3.4)a |
| SCNT-FOC + K | 2 | 109 | 103 (95) | 102 (99) | 1 (1 ± 1)b | 85 (83 ± 3.5)b |
| SCNT-CROC | – | 139 | 132 (95) | 93 (70) | 39 (30 ± 1.8)a | 30 (23 ± 3.1)a |
| SCNT-CROC + K | 2 | 130 | 125 (96) | 123 (98) | 2 (2 ± 1.2)b | 82 (66 ± 2.4)c |
SCNT-FOC, cloned embryos from somatic cell nuclear transfer using fresh oocyte cytoplasm; SCNT-CROC, cloned embryos from somatic cell nuclear transfer using cryopreserved (vitrified/warmed) oocyte cytoplasm; K, injection of lysine (K)-specific demethylase 4A (Kdm4a) mRNA. Within the same column, values with different superscript letters (a, b, c) are significantly different (p < 0.05; n = 5).
a
Based on the number of SCNT oocytes.
b
Based on the number of 2-cell embryos.