Table 2.
Effect of Kdm4a mRNA Injection and Melatonin Supplement on the Developmental Potential in Cloned Embryos of Cryopreserved Mouse Oocytes
| Group | Melatonin (μM) | No. of NT Oocytes | No. of 2-Cell Embryos (%)a | No. of 4-Cell Embryos (%)b | No. of 2-Cell Block Embryos (% ± SEM)b | No. of Blastocysts (% ± SEM)b |
|---|---|---|---|---|---|---|
| SCNT-CROC | – | 125 | 112 (90) | 66 (59) | 46 (42 ± 3.7)a | 23 (20 ± 1.5)a |
| SCNT-CROC + M | 10 | 125 | 114 (91) | 81 (71) | 33 (31 ± 3.7)a | 44 (39 ± 1.2)b |
| SCNT-CROC + K | – | 138 | 128 (98) | 124 (94) | 4 (3 ± 1.5)a | 76 (59 ± 3.3)a |
| SCNT-CROC + K + M | 10 | 138 | 123 (96) | 119 (96) | 4 (4 ± 1.8)a | 91 (75 ± 3.3)b |
SCNT-CROC, cloned embryos from somatic cell nuclear transfer using cryopreserved (vitrified/warmed) oocyte cytoplasm; M, treatment of melatonin; K, injection of lysine (K)-specific demethylase 4A (Kdm4a) mRNA (2 μg/μL). Within the same column, values with different superscript letters (a, b) are significantly different (p < 0.05; n = 5).
a
Based on the number of SCNT oocytes.
b
Based on the number of 2-cell embryos.