Figure 1.

High- and Low-CF NM Neurons in Slice Culture

(A) Development of chicken auditory system in vivo and in vitro.

(B) NM is organized tonotopically from rostromedial to caudolateral direction. Four to five slices of 200 μm thickness were prepared from an animal at E11 after retrograde labeling of NM neurons with dextran TMR. NM in rostral one or two slices was defined as high-CF (HCF) region and that in the caudal most slice as low-CF (LCF) region.

(C) Cultured slice at 14DIV. NM was labeled with dextran TMR (upper right). Bright-field (upper left) and fluorescence (lower left) images of the slice. Square was magnified (lower right). Anterogradely labeled projections from contralateral NM (arrowhead).

(D) Retrogradely labeled NM neurons in high- (upper) and low-CF regions (lower) at 1DIV (left) and 14DIV (right). Arrowheads indicate axon.

(E and F) Cross-sectional area (E) and the number of dendrites (F) of cultured neurons. Numbers in parentheses are the number of cells. **p < 0.01.

See also Figure S1.