Table 2.
Genes | Primer Sequences |
---|---|
PVT1 | F: 5′-TTGGCACATACAGCCATCAT-3′ |
R: 5′-GCAGTAAAAGGGGAACACCA-3′ | |
miR-424-5p | F: 5′- CAGCAGCAATTCATGT-3′ |
R: 5′- TGGTGTCGTGGAGTCG-3′ | |
U6 | F: 5′-GGCTGGTAAGGATGAAGG-3′ |
R: 5′-TGGAAGGAGGTCATACGG-3′ | |
CARM1 | F: 5′-TTGATGTTGGCTGTGGCTCTGG-3′ |
R: 5′-ATGGGCTCCGAGATGATGATGTCC-3′ | |
MMP-2 | F: 5′-AGGGCACATCCTATGACAGC–3’ |
R: 5′-ATTTGTTGCCCAGGAAAGTG–3’ | |
MMP-9 | F: 5′-GCCACCCGAGTGTAACCAT-3′ |
R: 5′-TCCCTGGAGACCTGAAACC-3′ | |
Bax | F: 5′-AAGCTGAGCGAGTGTCTCCGGCG-3′ |
R: 5′-GCCACAAAGATGGTCACTGTCTGCC-3′ | |
Bcl-2 | F: 5′-CTCGTCGCTACCGTCGTGACTTCG-3′ |
R: 5′-CAGATGCCGGTTCAGGTACTCAGTC-3′ | |
GAPDH | F: 5′-AGTCCACTG GCGTCTTCA-3′ |
R: 5′-GAGTC CTTCCACGATACCAA-3′ |
miR-424-5p, microRNA-424-5p; CARM1, co-activator-associated arginine methyltransferase 1; MMP-2, matrix metalloproteinase-2; MMP-9, matrix metalloproteinase-9; Bax, Bcl2-associated X protein; Bcl-2, B cell lymphoma-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse.