Figure 3.

Cell cycle regulation of CKS2 and CKS2-SSBP1 complex in HeLa cells by flow cytometry. (A) CKS2 signal in G₁-, S-, and G₂-phase of the cell cycle. (B) DNA histogram showing the six regions used for gating of data presented in panels C and D. (C) CKS2-SSBP1 PLA signal and DNA content in cells gated as indicated in panel B. (D) Relative CKS2-SSBP1 PLA signal in cells gated as indicated in panel B. PLA signal divided by DNA content is shown. (C, D) Data are shown relative to the first gate in G₁-phase (region 1). (A, C, D) Each point represents mean value of at three (A) and five (C, D) independent experiments. The bars represent standard error of the mean. *, statistically significant difference (P < .05) compared to second region in G₁-phase (region 2).