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. 2019 Mar 5;10:150. doi: 10.3389/fgene.2019.00150

Table 1.

Comparison of read assignment between full coverage poly-A RNA-seq, SFL, and 3′DGE.

Counts (million)
Percent (value/library size)
Mean (SD) Median Minimum Maximum Mean (SD) Median Minimum Maximum
Poly-A RNA-seq (RNA-seq)
Library size (total reads) 13.0 (2.3) 12.6 9.3 17.6
Aligned reads 12.4 (2.2) 12.0 9.0 16.9 95.9 (1.3) 96.0 92.4 97.9
Uniquely aligned reads 10.8 (1.9) 10.3 7.8 14.8 82.9 (1.5) 83.0 79.5 85.3
Counted reads   8.4 (1.5) 8.1 6.4 10.9 65.2 (2.7) 64.6 60.5 70.3
Sparse full length sequencing (SFL)
Library size (total reads)   3.8 (1.1) 3.5 1.6 6.9
Aligned reads   3.3 (1.0) 3.1 1.4 5.9 88.5 (2.9) 88.8 73.0 92.5
Uniquely aligned reads   1.8 (0.6) 1.8 0.7 3.2 48.5 (8.0) 46.8 27.6 64.8
Counted reads   0.9 (0.3) 0.9 0.3 1.6 24.5 (4.0) 23.8 14.3 31.7
3′ digital gene expression (3′DGE)
Library size (total reads)   3.7 (0.7) 3.7 1.9 5.6
Aligned reads   3.0 (0.6) 3.0 1.5 4.5 80.6 (1.6) 81.0 73.5 82.2
Uniquely aligned reads
Counted reads   1.2 (0.2) 1.2 0.7 1.8 33.3 (1.4) 33.0 30.5 38.6