Table 1.
Comparison of read assignment between full coverage poly-A RNA-seq, SFL, and 3′DGE.
Counts (million) |
Percent (value/library size) |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|
Mean (SD) | Median | Minimum | Maximum | Mean (SD) | Median | Minimum | Maximum | |||
Poly-A RNA-seq (RNA-seq) | ||||||||||
Library size (total reads) | 13.0 (2.3) | 12.6 | 9.3 | 17.6 | ||||||
Aligned reads | 12.4 (2.2) | 12.0 | 9.0 | 16.9 | 95.9 (1.3) | 96.0 | 92.4 | 97.9 | ||
Uniquely aligned reads | 10.8 (1.9) | 10.3 | 7.8 | 14.8 | 82.9 (1.5) | 83.0 | 79.5 | 85.3 | ||
Counted reads | 8.4 (1.5) | 8.1 | 6.4 | 10.9 | 65.2 (2.7) | 64.6 | 60.5 | 70.3 | ||
Sparse full length sequencing (SFL) | ||||||||||
Library size (total reads) | 3.8 (1.1) | 3.5 | 1.6 | 6.9 | ||||||
Aligned reads | 3.3 (1.0) | 3.1 | 1.4 | 5.9 | 88.5 (2.9) | 88.8 | 73.0 | 92.5 | ||
Uniquely aligned reads | 1.8 (0.6) | 1.8 | 0.7 | 3.2 | 48.5 (8.0) | 46.8 | 27.6 | 64.8 | ||
Counted reads | 0.9 (0.3) | 0.9 | 0.3 | 1.6 | 24.5 (4.0) | 23.8 | 14.3 | 31.7 | ||
3′ digital gene expression (3′DGE) | ||||||||||
Library size (total reads) | 3.7 (0.7) | 3.7 | 1.9 | 5.6 | ||||||
Aligned reads | 3.0 (0.6) | 3.0 | 1.5 | 4.5 | 80.6 (1.6) | 81.0 | 73.5 | 82.2 | ||
Uniquely aligned reads | ||||||||||
Counted reads | 1.2 (0.2) | 1.2 | 0.7 | 1.8 | 33.3 (1.4) | 33.0 | 30.5 | 38.6 | ||