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. 2019 Mar 5;10:361. doi: 10.3389/fimmu.2019.00361

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Copyright © 2019 Pararasa, Zhang, Tull, Chong, Siu, Guesdon, Chavele, Sanderson, Langmead, Kok, Spencer and Vossenkamper.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B cell subsets in GALT of healthy control and IBD patients. Data was gated on the Cytobank platform. Following selection of CD19⁺ cells and elimination of CD10⁺ cells (since these would include germinal center cells in tissues and therefore not be equivalent to CD10⁺ subsets in blood), cells were gated according to their expression of CD27 and IgD. Examples of a HC and an IBD sample are illustrated in (A). In (B) dotplots represent individually gated data points for HC (n = 6), patients with UC (n = 6 in blue) and a patient with CD in green, groups together as IBD. Groups were compared by Mann-Whitney U test where p < 0.05 = ^*.