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. 2019 Mar 5;10:361. doi: 10.3389/fimmu.2019.00361

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Copyright © 2019 Pararasa, Zhang, Tull, Chong, Siu, Guesdon, Chavele, Sanderson, Langmead, Kok, Spencer and Vossenkamper.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TNFα and IL-10 expression by blood and tissue B cells. Blood (PBMC) and GALT (CF, colonic follicles; PP, Peyer's patches) samples were obtained from HC (n = 7) and CD patients (n = 3) and isolated CD19⁺ cells were analyzed for (A) TNFα and (B) IL-10 expression according to the strategy in Supplemental Figure 4. Subsets of B cells were sorted from blood as in Supplemental Figure 3, and expression of (C) TNFα and (D) IL-10 measured. Data was analyzed by Kruskal Wallis ANOVA test, where p < 0.05 = ^* and p < 0.01 = ^**.