Table 1.

Kinetic parameters for (S)‐nicotine‐iminium and (S)‐nornicotine formation by variant CYP2B6 proteins

Allele	Protein variant	(S)‐iminium			(S)‐nornicotine			CLint (nornicotine/iminium)
		K m (μmol L−1)	V max (pmol min−1 pmol−1)	CLint (mL−1 min−1 nmol−1)	K m (μmol L−1)	V max (pmol min−1 pmol−1)	CLint (mL min−1 nmol−1)
*1		269 ± 37	0.85 ± 0.04	0.0032	184 ± 27	0.19 ± 0.01	0.0010	0.33
*4	K262R	333 ± 32	0.83 ± 0.03	0.0025	167 ± 28	0.21 ± 0.01	0.0013	0.50
*5	R487C	257 ± 22	0.66 ± 0.01	0.0026	132 ± 18	0.09 ± 0.00	0.0007	0.27
*6	Q172H/K262R	307 ± 16	0.58 ± 0.01	0.0019	110 ± 15	0.06 ± 0.00	0.0005	0.29
*7	Q172H/K262R/R487C	236 ± 21	0.56 ± 0.01	0.0024	108 ± 23	0.09 ± 0.00	0.0008	0.35
*9	Q172H	406 ± 44	0.18 ± 0.01	0.0004	134 ± 21	0.02 ± 0.00	0.0001	0.34
*17	T26S/D28G/R29T	332 ± 22	0.85 ± 0.02	0.0026	130 ± 17	0.11 ± 0.00	0.0008	0.33
*19	Q172H/K262R/R336C	271 ± 27	0.60 ± 0.02	0.0022	114 ± 26	0.06 ± 0.00	0.0005	0.24
*26	P167A/Q172H/K262R	189 ± 21	0.41 ± 0.01	0.0022	86 ± 20	0.08 ± 0.00	0.0009	0.43