Figure 1.

Phylogenetic relationships of the 13 algal species used in this study. Actin protein sequences of the species described in Table 1 were aligned by the MUSCLE alignment method and the phylogenetic tree was constructed by the Maximum-likelihood method using the MEGA6 software package. The tree represents the known phylogenetic relationships of the selected species, separation of the three major classes (Prasinophyceae, Trebouxiophyceae and Chlorophyceae) is evident. There is a distinct split within the marine picoeukaryotes of Prasinophyceae, where the two Ostreococcus species and B. prasinos form a separate clade.