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. 2019 Mar 11;10(3):238. doi: 10.1038/s41419-019-1485-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Immunohistochemical (IHC) staining of the basal/progenitor marker p63 and cell proliferation marker Ki67. The right images are magnifications of the boxes in the left image. The cells located at the basement membrane (a1, a1′) and sweat gland (a2, a2′) highly expressed p63 and Ki67. And the cells located at skin dermis (a3, a3′) did not express p63 and Ki67. b Immunofluorescence (IF) staining displayed the colocalization of epidermal cell markers (CK14, CK10, and CK5) with sweat gland cell markers (αSMA, SOX9, CK18, and CK19). The yellow dashed line represents the boundary between epidermis and dermis. The lower images show the respective magnification of the boxes in the upper images of the duct-connected epidermis (left) and the secretory gland under the deep dermis (right). The expression levels are summarized in the table. c Colocalization of sweat gland functional markers, αATP, AQP5, and CEA, with αSMA especially in the secretory portion of the sweat gland. Nuclei (blue) were counterstained with 4', 6'-diamidino-2-phenylindole (DAPI). Scale bars: 50 μm