Fig. 6. Transplantation of SGOs promoted wound healing and sweat gland regeneration in vivo.

a The normal mice with wound or injured sweat glands were used to monitor the therapy effect of tdTomato-autologous SGCs isolated from syngeneic mice. b Representative images of wounds in mice of two test groups for 0–21 days. c Dermal wound closure (%) in the different treatment groups, and the wound area over time was measured as a percent of the original area. d Wound sections on 3, 7, 14, and 21 days of post transplantation (DPT) were stained with H&E for general observation of skin layers. e Skin thickness and epidermal thickness on 14 and 21 DPT in the different treatment groups. f IF staining showed the colocalization of the epidermal-specific marker, CK14, with transplanted tomato-sweat gland organoids at day 21 after transplantation in the cell-treated group. g Iodine/starch-based sweat test on paws of mice before injury and at days 0, 7, 14, and 21 after transplantation. Dark spots on foot paw pads were positive in cell-treated group. h The distribution of tdTomato-SGCs after transplantation, and the histology of sweat gland regeneration in mouse paws at days 3, 7, 14, and 21. i IF staining showed the colocalization of sweat-gland-specific marker, CK19, and functional marker, AQP5, with transplanted tdTomato-SGOs at day 21 after transplantation in the treated group. **P < 0.01. Scale bars: d 400 μm; f, h, i 100 μm. SGO sweat gland organoid, SGC sweat gland cell, IF immunofluorescence