Fig. 5.

The interaction between transcription bubble and RNAP in the Mtb σ^H-RPo structure. a Recognition of the “G₋₁₁T₋₁₀T₋₉” sequence in the −10 element by σ^H₂. b Stacking of −7(nt) and −6(nt) nucleotides by RNAP-β subunit and σ^H₂. c The recognition of G₋₅(nt) by RNAP-β subunit and σ^H₂. d The interaction between RNA polymerase (RNAP) and CRE element. Colors are as above. e The interaction between RNAP and DNA/RNA hybrid. f The in vitro transcription activity of RNAP derivatives comprising alanine substitutions of DNA-contacting residues on RNAP-β subunit and σ^H. The experiments were repeated in triplicate, and the data are presented as mean ± S.E.M. Source data of f are provided as a Source Data file