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. 2019 Mar 11;10(3):236. doi: 10.1038/s41419-019-1487-3

Fig. 3. NAA40 knockdown reduces CRC cell growth in vitro.

Fig. 3

a MTT cell viability assay of SCR and NAA40-KD HCT116, HT-29, SW480, or SW620 cells incubated with various concentrations of dox. Cell viability is shown as a percentage relative to the corresponding dox untreated cells. The data are shown as mean ± s.d. of three replicates. Unpaired two-tailed Student’s t-test was used (*p < 0.05, **p < 0.01, ***p < 0.001 compared to SCR control cells). b Phase contrast microscopy of dox-inducible HCT116, HT-29, SW480, and SW620 engineered cell lines. Dashed rectangles indicate the zoomed-in areas shown in the right panels. Cells were captured in at least five fields of view (×100 magnification). The images are representative microscope fields from at least three independent replicates. Scale bar, 100 μm. c MTT assay of SCR and NAA40-KD HCT116 (left) and HT-29 (right) cells treated with DMSO, dox, 5-FU, or dox + 5-FU. Cell viability is presented as a percentage relative to the corresponding untreated cells. Error bars represent the mean ± s.d. of three replicates. Unpaired two-tailed Student’s t-test was used to compare 5-FU treatment alone to the treatment of 5-FU in combination with dox (ns = no significance, **p < 0.01, ***p < 0.001)