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. 2019 Mar 11;9:4055. doi: 10.1038/s41598-019-40640-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of odorant induced EAG responses of fruit fly antennae by an Orco antagonist. (A) Top, EAG recordings from a single insect exposed sequentially to stimulus pulses of air, 0.01% EA, 0.1% EA and 1% EA. The stimulus pulse set was repeated with the main airflow containing air, air + 50% LF, air, air + 50% BMP, air. Bottom, graphic representation of repetitions (n = 3–5) of the experiments shown above. Data are presented as mean ± SEM. (B) Top, EAG recordings from a single insect exposed sequentially to stimulus pulses of air, 0.01% 2 H, 0.1% 2 H and 1% 2 H. The stimulus pulse set was repeated with the main airflow containing air, air + 50% LF, air, air + 50% BMP, air. Bottom, graphic representation of repetitions (n = 3) of the experiments shown above. Data are presented as mean ± SEM. Statistical analysis: The stimulus pulses of each type (air, 0.01% EA, etc.) were compared by one-way ANOVA and Dunnett’s multiple comparison test. For comparison to the stimulus pulse in the first main airflow set (Air): a, p < 0.05; b, p < 0.01; c, p < 0.001; d, <0.0001.