Table 1.

Primers used for generation of mutants.

Primer	Sequence (5′– 3′)	Reference
DUS_UHS_fw	ATGCCGTCTGAAAATTAAGTTAGAATTATCCCTAT	This work
UHS_ldhA_rev	TTTCCATTTCAAAACAAATCCAAAATCATACTGCCATAATT	This work
ldhA_Cm_DHS_fwd	CCAACTTGGCAACATGCCGTCTGAATGAGACGTTGAT	This work
DHS_rev	TTGCTAACAGAAAACTCTACTCC	This work
UHS_ldhA_fwd	ATGATTTTGGATTTGTTTTGAAATGGAAATGCCGTGCA	This work
ldhA_Cm_DHS_rev	CATTCAGACGGCATGTTGCCAAGTTGGAAGTGA	This work
ldhA_up_fwd	ATGCCGTCTGAATTCAGTGTATTATGCCGT	Sigurlasdottir et al., 2017
ldhA_dn_rev	TAAAACACGTCAGCCGTCG	Sigurlasdottir et al., 2017

The DNA uptake sequence is marked in italic. The overlapping sequences for fusion PCR are underlined.