Table 2.

qPCR primers used in the study.

Primer	Sequence (5′– 3′)	Reference
aniA_qPCR_fw	AGGCGAAACCGTGCGTATGT	This work
aniA_qPCR_rev	GGAAGACACTAGGTTCGGAC	This work
narP_qPCR_fw	GACCGCCAAACTCGTTAAAAG	This work
narP_qPCR_rev	GAGATAGCCCAAGATTTCCAG	This work
siaD_qPCR_fw	CCTACTACCCAATGTCTGTCAA	This work
siaD_qPCR_rev	GCTCTTCAATTAAAGCGGTGTTC	This work
pilE_qPCR_fw	TATTCCGACAACGGCACATTCCC	Kuwae et al., 2011
pilE_qPCR_rev	CCTTCAACCTTAACCGATGCCA	Kuwae et al., 2011
pilX_qPCR_fw	CGGGGACGGGTTATACTTT	Sigurlasdottir et al., 2017
pilX_qPCR_rev	GGCATCACGGCATTTGTATC	Sigurlasdottir et al., 2017
nalP_qPCR_fw	AGTCTCGCCGCTACCGTCTAT	This work
nalP_qPCR_rev	CCACTTTCAGCAGTTTGCCCA	This work
nhbA_qPCR_fw	AGATGCCGCTGATTCCCGTCAA	This work
nhbA_qPCR_rev	TTTTCCGCCCCGTAAGTCAGA	This work
mltA_qPCR_fw	CGAGCATCCGTATGTTTCCATC	This work
mltA_qPCR_rev	ATAAGACTTAATGCCCTGCATGG	This work
mltB_qPCR_fw	ATTATGACGGGGACGGACATC	This work
mltB_qPCR_rev	GCCAATGATTGCCTGAACAT	This work
ampD_qPCR_fw	CTTCATTCGGCGGCAGGGAAAA	This work
ampD_qPCR_rev	CAGATTGCGTCCAACAAGGCT	This work
rpsJ_qPCR_fwd	TTGGAAATCCGCACCCACTT	Kuwae et al., 2011
rpsJ_qPCR_rev	TACATCAACACCGGCCGACAAA	Kuwae et al., 2011