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. 2018 Dec 19;47(5):2546–2559. doi: 10.1093/nar/gky1266

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Veneno accumulates in cytoplasmic Ven-bodies. (A) Schematic representation of Ven transgenes used in IFA experiments. (Full: amino acid [aa] 1–785; C91: aa 91–785; C199: aa 199–785; C234: aa 234–785; C400: aa 400–785; N670: aa 1–670; N399: aa 1–399; red lines indicate sequences of low amino acid complexity, rich in asparagines [N] or glutamines [Q]). (B–H) Representative confocal images of Aag2 cells expressing transgenes drawn schematically in (A). Scale bar represents 10 μm. (I) The cytoplasm of 46–56 individual cells expressing GFP-tagged transgenes was traced as depicted and the mean and standard deviation of signal intensity was determined to calculate the coefficient of variation as a measure of signal granularity. (J) Scatter dot plot shows the GFP-signal granularity for individual cells; the red lines indicate the mean.