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. 2018 Dec 21;47(5):2402–2424. doi: 10.1093/nar/gky1279

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — LIG1 is required for 17p sister chromatid telomere fusions. (A) Fusion amplicons derived from 17p or 21q TALEN (TLN) or control (0 DNA) -transfected unsynchronized or G2-arrested L1^−/fx:Cre were visualized by Southern hybridization using probes specific for the 17p (upper panels) or 21q subtelomeric (lower panels) sequence. A 2.9 kb constitutive amplicon band of known sequence identity is also detected with the 21q probe, but excluded from all fusion amplicon analyses. (B) Summary of 17p telomere fusion frequency (fusions/ng DNA input x10⁻²) data from seven independent 17p TLN and control (C) transfection experiments in unsynchronized (U) and G2-arrested (G2) L3^−/−:L4^−/-and L1^−/fx:Cre cells displayed as mean values with 95% confidence intervals with one-tailed paired T-tests or Mann–Whitney U-tests, as appropriate. (C) Box and whiskers representation of 17p telomere fusion frequency data presented in (b) as a pooled data comparison of all 17p TALEN-transfected L3^−/−:L4^−/− (grey) samples with all MeOH-treated L1^−/fx:Cre (black) samples and compared by two-tailed Mann–Whitney U-test. (D) Summary of 21q telomere fusion frequency (fusions/ng DNA input ×10⁻²) data from 3 independent 21q TLN and control (C) transfection experiments in G2-arrested L3^−/−:L4^−/-and L1^−/fx:Cre cells displayed as mean values with standard deviation and one-tailed paired T-tests or Mann–Whitney U-tests, as appropriate. (E) Southern hybridization images of G2-arrested WT 17p (upper panel) and 21q (lower panel) fusion amplicons generated as in (a). Untransfected (t0) and control (GFP) transfections are included for comparison with 17p and 21q TLN-transfected samples.