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. 2018 Dec 21;47(5):2402–2424. doi: 10.1093/nar/gky1279

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Ligase-depleted cells retain inter-chromosomal fusion capacity. (A) Southern hybridization detection of 17p fusions amplified from control (0 DNA) and 17p TLN-transfected unsynchronized and 4-OHT-treated L3^−/−:L4^−/−L1^−/fx:Cre (clones C15-5 and C15-9) and parental clone C15 (L3^−/−:L4^−/−L1^−fx) cells harvested after 48 h. Corresponding DNA content for each sample at the point of transfection is displayed below as linear histograms with annotated ploidy peaks. (B) Southern hybridization detection of 21q fusions induced by transfection of G2-arrested and 4-OHT-treated L3^−/−:L4^−/−L1^−/fx:Cre and L3^−/−:L4^−/−L1^−fx cells with 17p and 21q TLN over 48 h. Transfection efficiency (%) of each sample at 24 h post-nucleofection is indicated in below the lanes. (C) Bar chart summary of 17p (left side) and 21q (right side) fusion frequency data displayed in (A) and (B) for parental L3^−/−:L4^−/−L1^−fx clone C15 and L3^−/−:L4^−/−L1^−/fx:Cre clones C15-5 and C15-9 in the presence of MeOH or 4-OHT.