Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 11;9:4086. doi: 10.1038/s41598-019-40608-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Automated segmentation of cell bodies aided by speckle reduction. (a) En face plane extracted from a single depth in a single volumetric image. The imaged NIH-3T3 fibroblast exhibits significant speckle artifacts, which hinder automated segmentation. Arrow indicates cellular structure not retained by our segmentation procedure (see text for details). (b) The same En face plane as in (a), after combining eight sequential volumetric acquisitions. Speckle contrast is reduced, allowing for segmentation via K-means clustering. (c) Summation projection of the 3D segmented volume, which approximates the cell body. (d) 3D mesh of the cell body, generated from the data depicted in (c). Note that (d) was generated from a different viewing angle as (a–c) to more clearly depict the cell’s 3D shape.