Table 2.
Primers used for real-time-PCR.
| Gene | Primer name | Primer sequence | Reference |
|---|---|---|---|
| rpoA | qPCR_rpoA_F qPCR_rpoA_R |
GGCGCTCATCTTCTTCCGAAT CGCGGTCGTGGTTATGTG |
Modified based on ref.63 |
| espB EPEC | qPCR_EspB_F qPCR_EspB_R |
GGCTCTTTTGCTGCCATTAATAGC TCTGCTGCATCTGCAATACC |
This study |
| espA EPEC | qPCR_EspA_F qPCR_EspA_R |
GTGCGAATGCGAGTACTTCGAC TTGCAGCCTGAAAAACACCGAGT |
This study |
| tir EPEC | qPCR_Tir_F qPCR_Tir_R |
GGACCCTCTGCATTTCGTGTTG GTCCCCCGGTAAAAACAAATCTG |
This study |
| escJ EPEC | qPCR_EscJ_F qPCR_EscJ_R |
GCAAGCACTGTTGCTATCCA GCTGGGTGGGAAAATAACCT |
This study |
| espB EHEC | qPCR_EspBH_F qPCR_EspBH_R |
GCTTCGGAGAGTACGACCG
CGGCCTGCTGAATCTGATAGC |
This study |
| espA EHEC | qPCR_EspAH_F qPCR_EspAH_R |
CCACGGCACAAAAGATGGC
CCGCCTTCACTGTTTGCAG |
This study |
| tir EHEC | qPCR_TirH_F qPCR_TirH_R |
CGCCTGTAAGGAATTCTATGGC
GCCTGTTAAGAGTATCGAGCGG |
This study |
| sipB | qPCR_SipB_F qPCR_SipB_R |
GGAACAAAGTCCGGCGAGAG
TGAGACAGCGAAACATCGCC |
64 |
| sipC | qPCR_SipC_F qPCR_SipC_R |
AAACCCAGTTACGCGAGCAG
TGCCGTCGTTTTAGCTGCAT |
64 |
| avrA | qPCR_AvrA_F qPCR_AvrA_R |
TGTTGAGCGTCTGGAAAGTG
CAGATTCAACGCCTTCCATT |
65 |