Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 11;9:4066. doi: 10.1038/s41598-019-39343-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

IFNβ impacts endogenous SKP2 in HeLa S3 cells. (A) HeLa S3 cells were treated with IFNβ (500 pM) for the time indicated and lysed. Levels of endogenous SKP2 and USP18 were analyzed by western blot of whole cell lysates. (B) HeLa S3 cells were transfected with control siRNA (siCTRL) or siRNA targeting USP18 or ISG15. Cells were treated with IFNβ for 24 h and compared with untreated cells. Lysates were analyzed by western blot with anti-SKP2, anti-USP18 or anti-ISG15 antibodies. IFIT1 protein was monitored as control of IFN stimulation. Boxed membranes: 20 x less cell lysate was used. SKP2 band was normalized to GAPDH. Results are reported as the ratio of SKP2 to GAPDH. The ratio obtained for unstimulated cells was set to 1. Long exposure: time extended 3×, after hiding the right portion of the membrane.