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. 2019 Mar 11;9:4159. doi: 10.1038/s41598-019-40128-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Synaptophysin expression in Tuj1-positive cells grown in the presence and absence of an ECM coating. Representative fluorescent images showing Tuj1-positive neurons (red) and synaptophysin expression (green) at 15 DIV (a) and 30 DIV (b). Scale bar = 100 μm. Bar graphs summarize the integrated density of Tuj1 (c) and synaptophysin normalized to Tuj1 (d) per field of view. Data are expressed as a fold change relative to PDL group at 15 DIV, as shown by the dash line, presented as mean ± SEM for the number of cultures indicated at the base of each bar (n = 3–4 biological repeats), and were analyzed using two-way ANOVA with Tukey’s post hoc test.