Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 11;9:4157. doi: 10.1038/s41598-019-40612-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Immunocytochemical analysis of individual cells captured on the LoaD platform. (a,b) Representative immunocytochemical analysis of PECAM-1, ICAM-1 and E-selectin expression in individual HUVECs and EA.hy296 cells following treatment of cells with or without TNF-α (20 ng/ml) or LPS (10 ng/ml) for 24 hours. Bright field images and corresponding fluorescent images taken at 20x magnification with uniform region of interest selection for each image. (c,d) Cumulative analysis of the photonic fluorescence counts for PECAM-1, E-selectin and ICAM-1 following treatment with TNF-α and LPS, respectively. (e,f) Inhibition of TNF-α induced endothelial activation of PECAM-1 ICAM-1 and E-selectin in individual HUVECs and EA.hy296 cells, respectively, following NFκB inhibition with IκBα. (N = 3, *p = 0.035 ^#p = 0.04 HUVECs, *p = 0.045 ^#p = 0.04 EA.hy926 cells, n = 120 cells).