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. 2019 Mar 11;9:4157. doi: 10.1038/s41598-019-40612-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Double stained adherent endothelial progenitor cells (EPCs). Fluorescence microscopy (100x objective, bar corresponds to 20 µm) illustrates double-labelled adherent cells viewed in bright field (a). The cells were positive for binding of FITC-Ulex-lectin (b), and for uptake of Dil-labeled acetylated LDL (c). The staining in (d) show that the same cells stained for both FITC-Ulex-lectin and acetylated LDL and therefore agree with the current definition of cultured endothelial progenitor cells.