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. 2019 Mar 11;10(3):240. doi: 10.1038/s41419-019-1486-4

Fig. 5. ADI1 increased SAMe levels and suppressed CAV1 expression by altering promoter methylation status.

Fig. 5

a Quantification of SAMe levels in Huh7 cells with knockdown (KD) or complementary extraneous expression (OE) of ADI1. b Quantification of SAMe levels in Huh7 cells transiently expressing wild type (WT) or mutant ADI1s. P values were calculated by unpaired two-tailed Student's t-test. *p < 0.05, **p < 0.01, ***p < 0.001. c Western blot analysis of lysates from Huh7 or J7 cells pre-treated with SAMe in indicated concentrations. d The bisulfite converted CAV1 promoter region was analyzed by next-generation sequencing in J7 cells with or without ADI1 overexpression (OE). The percentage of methylated CpG islands were labeled. TSS, transcription start site. Western blot and RT-qPCR analysis of samples from empty vector transfected or ADI1-expressing e Huh7 or f J7 cells pre-treated with or without DNMT inhibitor. The short vertical bars represented standard deviations