Supplementary Figure S1.

RNF126 recruitment to the DNA damage stripe is dependent on PARP1 and ATM A. U2OS cells transiently expressing GFP-RNF126 were pre-treated with mock control (DMSO), ATM inhibitor KU55933, ATR inhibitor NU6027, PARP inhibitor olaparib, or DNA-PKcs inhibitor NU7026 for 1 h before subjected to micro-irradiation with a 365-nm UV laser beam (white dashed line). Images were collected every 30 s after irradiation and representative images are shown. B. The percentage of cells positive for GFP-RNF126 enrichment at DNA damage stripes was determined by analyzing >100 GFP-positive cells for each treatment. Data represent the mean ± SD. A two-way ANOVA was performed. **P < 0.01. ns, not significant. Scale bar, 10 μm. ATM, ataxia telangiectasia mutated; ATR, ataxia telangiectasia and Rad3-related protein; DNA-PKcs, DNA-dependent protein kinase catalytic subunit; PARP1, poly [ADP-ribose] polymerase 1.