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. 2018 Dec 19;7(3):597–618. doi: 10.1016/j.jcmgh.2018.12.004

Figure 13.

Figure 13

Downstream targets and upstream activators of STK25. (A) Liver protein lysates from high-fat–fed mice treated with GalNAc-Stk25 ASO vs GalNAc-control ASO (12.5 mg/kg/wk) were analyzed by Western blot using antibodies specific for total ACC or phospho-ACC (Ser79), with pan-actin used as a loading control. (B and C) Liver lysates were collected from male C57BL/6J mice (B) fed a normal chow diet and fasted for 12 hours vs re-fed or (C) fed a normal chow diet vs a high-fat diet for 18 weeks. The lysates were immunoprecipitated with anti-STK25 antibody and analyzed by Western blot using antibodies specific for total STK25 or phospho-threonine. Immunoprecipitation of liver lysates from Stk25 knockout mice was included as a negative control. Protein levels were analyzed by densitometry and data are shown as the total and phospho-protein abundance, as well as the ratio of phospho-protein to total protein. Representative Western blots are shown. Data are means ± SD from 6–8 mice per group. Cntr, control. *P < .05.