Figure 1.

CBX8 robustly associates with chromatin via its chromodomain. (A) Genome-wide analysis of CBX8 and H3K27me3 peak overlaps in K562, (47) Hs68, (42) and BF (42) cell lines, respectively. Numbers indicate number of called peaks, overlap percent of total CBX8 peaks. (B) Analysis of CBX8 and H3K9me3 overlaps in K562 cells. (C) CBX8 and H3K4me3 overlaps in K562, Hs68, and BF cells, respectively. (D) Sequential salt extraction in T98G (left) and HEK293T (right) cells examining endogenous CBX paralog chromatin association. Paralog elution patterns were detected using immunoblot and antibodies against the respective paralog. (E) Anti-V5 co-immunoprecipitation of WT and ΔCD CBX8, with detection of the PRC1 subunit BMI-1 to verify PRC1 complex incorporation. (F) Representative SSE of sgControl T98G cells (anti-CBX8), wild type and ΔCD re-expression (anti-V5) in T98G sgCBX8 cells. (G) Quantitation of SSEs displayed in (F), sgControl n = 3, WT and ΔCD n = 4 biological replicates, errors bars represent standard error of the mean (SEM), P-values were calculated using Student's two-tailed t-test: *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001