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. 2018 Dec 28;47(5):2666–2680. doi: 10.1093/nar/gky1299

Figure 2.

Figure 2.

Environmental influence on the structure of S. typhimurium H-NS. (A) Structural models for site1 (residues 1–57 are shown). Top: antiparallel model, as observed in E. coli H-NS1–46 (1NI8, NMR), S. typhimurium H-NS1–83,C21S (3NR7, X-ray), S. typhimurium H-NS1–46 (4ICG, X-ray) and V. cholerae VicH1–48 (1OV9, X-ray). bottom: parallel model, as determined for S. typhimurium H-NS1–57,C21S (1LR1, NMR). The two chains of each dimer are coloured green and cyan. (B) Superimposed are SAXS data collected on S. typhimurium H-NS2–57,C21S at 10°C (light blue), 20°C (dark blue), 30°C (green) and 42°C (red). Data were collected in 150 mM NaCl, 1 mM EDTA, 20 mM Tris pH 7.5. (C) Pair distance distributions P(r) calculated for the parallel (green) and antiparallel (red) models for H-NS1–57,C21S. The encircled area and red arrow highlight a main difference between the both site1 models. (D) P(r) for H-NS2–57,C21S, derived from SAXS data shown in (D); 20°C (blue), 30°C (green) and 42°C (red). (E) Fit of calculated SAXS pattern (green) to experimental data (red dots) collected at 10°C. Green: antiparallel model; χ2 = 2.22. Blue: parallel model; χ2 = 7.56. Fits were calculated by FoXS (57). (F–H) DLS measurements of RH as a function of temperature. (F) Temperature and concentration dependence on multimerization of H-NS without fused mCherry. Wt: full-length H-NS; ΔS2: H-NSΔs2. (G) Influence of salt on multimerization of mChH-NS (wt) and mChH-NSΔs2 (ΔS2). (H) as (G) but with variations in pH. For other salt and pH scans, please see Supplementary Figure S3. Data in (F–H) are means ± S.D., n = 3.