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. 2018 Dec 28;47(5):2666–2680. doi: 10.1093/nar/gky1299

Figure 4.

Figure 4.

H-NSΔs2 adopts a relatively compact conformation in vitro. (A) Experimental H-NSΔs2 SAXS patterns recorded at 50 mM NaCl (green) and 750 mM NaCl (pink). Calculated SAXS pattern from ensemble models are shown in black. The corresponding Guinier plots are shown in Supplementary Figure S8. (B) Histogram of Rg and Dm for pool (black line) and selected ensemble models (olive for 50 mM NaCl data, and pink for 750 mM NaCl data). a.u: arbitrary units. The corresponding ensemble models are shown in Supplementary Figure S8. (C) Rg and Dm values calculated from (A) and (B) are tabulated. (D) Interactions of fluorescently labeled H-NS84–137 with H-NS1–57 and 13bp DNA determined using MST. (E) Influence of NaCl on the H-NS84–137:H-NS1–57 interaction determined using MST. Data in (D, E) are means ± S.D., n = 3.