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. 2018 Dec 28;47(5):2666–2680. doi: 10.1093/nar/gky1299

Figure 6.

Figure 6.

H-NS:DNA interactions are incompatible with the H-NS N/C association. Interactions between H-NSFL and H-NSΔS2 with 101bp DNA were determined using (A) MST and (B) fluorescence anisotropy. (C) Influence of NaCl on the interaction between H-NSFL with 101bp DNA, determined using MST. Data in (A–C) are means ± S.D., n = 3. (D) Nuclease assay of lambda DNA in the presence of H-NSFL and H-NSΔS2 under different conditions. (E) Proposed temperature switch mechanisms. Top: Under conditions where site2 dimers are stable, H-NS will multimerize. In this conformation, the linker residues (magenta), DNAbd (yellow) and positive charges on site1 (stick representation) of linked H-NS molecules can cooperate to achieve a strong association with DNA (indicated as dashed line). Bottom: Under conditions where site2 unfolds, the C-terminal linker and DNAbd associate with the N-terminal site1, leading to an autoinhibited dimeric conformation incapable of DNA binding and of formation of higher-order multimers. Although based on experimental data, the positions of the acidic linker and DNAbd are speculative.