Table 1.
Genes associated with IPD.
| Gene | Length (bp) | Best matches, identity (%), e-value, Accession number | Information |
|---|---|---|---|
| hpp1 | 452 | 1. phtB, 9E-132, 447/465 (96%), NCBI, AF318954.1 2. phpA, 0, 451/480 (94%), NCBI, AF340221.1 |
1. PHT proteins (aka BHV) are thought to be involved in the invasion process of pneumococci59,60. 2. The PhpA protein elicits protective immune response against bacteremia and nasopharyngeal carriage in mice59. |
| hpp2 | 330 | hypothetical protein (CPS), 4E-168, 328/330 (99%), NCBI, JQ653094.1 | This is a putative capsular polysaccharide biosynthesis protein, Capsular differences are known to be associated with invasive disease7. |
| hpp3 | 249 | hypothetical protein | |
| hpp4 | 504 | phtD, 0, 504/504 (100%), NCBI, KP127799.1 | The found phtD hit was a part of a sequence shown to be highly conserved in invasive isolates61. |
| hpp5 | 954 | Hypothetical protein (CPS), 0, 954/954 (100%), NCBI, HE651314.1 | This is a putative capsular polysaccharide biosynthesis protein. Capsular differences are known to be associated with invasive disease7. |
| hpp6 | 996 | Hypothetical protein | |
| hpp7 | 231 | pspC, 1E-67, 167/179 (93%), NCBI, AF154043.2 | pspC was shown to be involved in immune response to bacteremia in mice36. |
| hpp8 | 510 | Hypothetical protein | |
| hpp9 | 324 | Hypothetical protein (CPS), 2E-161, 320/324 (99%), NCBI, ADM91299.1 | This is a putative capsular polysaccharide biosynthesis protein. Capsular differences are known to be associated with invasive disease7. |
| hpp10 | 504 | pspC,0, 502/504 (99%), NCBI, AF154022.1 | pspC was shown to be involved in immune response to bacteremia in mice36. |
| hpp11 | 306 | Hypothetical protein | |
| hpp12 | 399 | Hypothetical protein | |
| hpp13 | 327 | Hypothetical protein (CPS), 0, 511/528 (97%), NCBI, AF316639.1 | This is a putative capsular polysaccharide biosynthesis protein. Capsular differences are known to be associated with invasive disease7. |
| ydcP_1 | 471 | putative protease YdcP, 0, 470/471(99%), NCBI, AFS43444.1 | YdcP is part of the U32 protease family. It is a collagenase, facilitating breaking of extracellular structures tissues, and is a known virulence factor in other bacterial species62. |
| hpp14 | 519 | Hypothetical protein (CPS), 0 509/519 (98%), NCBI, AF154022.1 | This is a putative capsular polysaccharide biosynthesis protein. Capsular differences are known to be associated with invasive disease7. |
| hpp15 (hmo) | 147 | L-lactate dehydrogenase (FMN-dependent)-like/alpha-hydroxy acid dehydrogenase, 4E-70, 147/147(100%) | Lactate dehydrogenase was found to be essential enzyme for pneumococcal survival in blood63. |
| hpp16 | 480 | Hypothetical protein | |
| lytB | 1977 | Putative endo-beta-N-acetylglucosaminidase, 0, 1968/1977 (99%), NCBI, AJ870414.1 | lytB codes for a endo-beta-N-acetylglucosaminidase, which is responsible for cell-wall hydrolysis and is thought to be a virulence factor27,28. |
| hpp17 | 528 | Hypothetical protein (CPS), 511/528 (97%), NCBI, JF301964.1 | This is a putative capsular polysaccharide biosynthesis protein. Capsular differences are known to be associated with invasive disease7. |
| hpp18 | 516 | pspC, 508/516 (98%), NCBI, AF154043.2 | pspC was shown to be involved in immune response to bacteremia in mice36. |
| hpp19 | 489 | Hypothetical protein | |
| hpp20 | 387 | Hypothetical protein (partial transposase), 0, 387/387(100%), NCBI, ADM91518.1 | Part of the mobile genetic elements of the bacterium. |
| hpp21 | 258 | Hypothetical protein | |
| hpp22 | 288 | Hypothetical protein | |
| hpp23 | 210 | Hypothetical protein | |
| hpp24 | 387 | Hypothetical protein (partial transposase), 0.0, 386/387(99%), NCBI, CP002176 (positions 1374937–1375323) | Part of the mobile genetic elements of the bacterium. |
| hpp25 | 510 | Hypothetical protein | |
| hpp26 | 168 | Hypothetical protein | |
| hpp27 | 489 | pspC, 0, 463/490 (94%), NCBI, AF154022.1 | pspC was shown to be involved in immune response to bacteremia in mice36. |
| lox | 1137 | Lactate oxidase (lox) gene, 0, 1001/1137(88%), NCBI, DQ984140.3 | The lox gene is involved in bacterial niche competition and virulence in streptococci and other bacterial species30,31. |
| hpp28 | 840 | Sortase (srtA), 0, 614/740 (83%), NCBI, KX147105.1 | In Streptococcus mutans, disruption of the sortase (srtA) gene led to decrease in adherence and invasion to endothelial cells64. |
| hpp29 | 189 | Hypothetical protein | |
| hpp30 | 537 | Hypothetical protein | |
| hpp31 | 504 | Hypothetical protein | |
| hpp32 | 309 | Hypothetical protein | |
| hpp33 | 309 | Hypothetical protein | |
| cpsA | 1446 | cpsA (aka wzg), 0, 1446/1446 (100%), NCBI, KC522490.1 | wzg (aka cpsA) is part of the capsular polysaccharide synthesis gene locus. High expression of cpsA is associated with bacteremia in humans65. |
| bgaA | 6702 | bgaA (Beta-galactosidase BoGH2A), 6466/6704 (96%), NCBI, AF282987.1 | bgaA is hypothesized to be a pneumococcal virulence factor66 and was shown to promote resistance to immune cells in human serum67. |
| cpsA | 1446 | cpsA (aka wzg), 0, 1446/1446 (100%), NCBI, KC522492.1 | wzg (aka cpsA) is part of the capsular polysaccharide synthesis gene locus. High expression of cpsA is associated with bacteremia in humans65. |
| hpp34 | 207 | Hypothetical protein | |
| hpp35 | 573 | pspC, 0, 566/573 (99%), NCBI, AF154043.2 | pspC was shown to be involved in immune response to bacteremia in mice36. |
| hpp36 | 684 | cpsD, 0, 682/684 (99%), NCBI, AFC94091.1 | cpsD mutations were shown to inhibit the possibility of causing bacteremia in mice68. |
| hpp37 | 840 | Hypothetical protein |