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. 2019 Mar 11;9:4110. doi: 10.1038/s41598-019-40886-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Apoptosis and metabolic assay. (a) Annexin-V FITC apoptosis assay on control and Neo1-siRNA treated cells showed no significant difference in apoptotic cells after Neo1 knockdown (i) Unstained cells (ii) Control cells with no siRNA treatment (iii) Control siRNA treated cells (iv) Neo1 siRNA treated cells (b)% of apoptotic cells p-value = 0.07. (c) AQueous One metabolic assay showed a significant decrease in the metabolic activity of cells after Neo1 knockdown. p-value = 0.01.